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, 09:52, 15 February 2012
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| By checking the first graph "Empirical vs reported Phred score", we found reported base qualities are shifted to the right. | | By checking the first graph "Empirical vs reported Phred score", we found reported base qualities are shifted to the right. |
− | Further we notice that effects is caused by different software from Illumina sequencers.
| + | In this particular example, '33' was incorrectly added to all base qualities. |
− | In this particular example, '33' was incorrectly added to all base qualities. Such data used in variant calling may increase false positive SNP calling. | + | When such data used in variant calling, we may increase false positive SNP variants. |
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