MutationFilter

Introduction

• The tool mutfilter generates various diagnosis statistics based on sequence alignment and filters alignment artifacts based on user-provided criteria.
• It takes as input a SAM/BAM file (through --bam) and a BED-like file (through --bed) and generates output on screen.
• Additional filtering options can be provided and mutfilter will generate filtering flags for each input filtering option. See details below.

Usage

• Typing mutfiler without any other options will display the following message

The following parameters are available.  Ones with "[]" are in effect:
             Input Files : --bam [], --bed []
         Cycle Bias (CB) : --mut_median_cycles2ends [-1]
        Strand Bias (SB) : --SB_OR [-1.0e+00]
   Nearby Indels (INDEL) : --indel_winsize [30], --indel_cnt [-1],
                           --indel_pct [-1.0e+00]
          Head Clip (HC) : --mut_clip_cnt [-1], --mut_clip_pct [-1.0e+00]
      Other Alleles (OA) : --other_allele_cnt [-1],
                           --other_allele_pct [-1.0e+00]
    Allelic Balance (AB) : --mut_base_cnt [-1], --mut_base_pct [-1.0e+00]
   Low Map Quality (LMQ) : --low_mapq_cutoff [-1.0e+00], --low_mapq_cnt [-1],
                           --low_mapq_pct [-1.0e+00], --mut_low_mapq_cnt [-1],
                           --mut_low_mapq_pct [-1.0e+00]
  Low Base Quality (LBQ) : --low_baseq_cutoff [-1.0e+00],
                           --low_baseq_cnt [-1], --low_baseq_pct [-1.0e+00],
                           --mut_low_baseq_cnt [-1],
                           --mut_low_baseq_pct [-1.0e+00]
    Improper Paired (IP) : --improper_paried_cnt [-1],
                           --improper_paried_pct [-1.0e+00],
                           --mut_improper_paried_cnt [-1],
                           --mut_improper_paried_pct [-1.0e+00]

NOTE: When parameters are negative these filters are NOT in effect!
     When filters have 'mut_' the filters are for the statistis calculated for MUTANT alleles only!

• Explanation of all options