Changes

From Genome Analysis Wiki
Jump to navigationJump to search
Line 25: Line 25:     
*Assumes the file is sorted by Coordinate (or ReadName if using <code>--readName</code> option)
 
*Assumes the file is sorted by Coordinate (or ReadName if using <code>--readName</code> option)
*Assumes only 2 reads have matching ReadNames
+
*Assumes only 2 reads have matching ReadNames (Supplementary and Secondary reads are ignored/skipped by default so will not cause a problem)
 
**It matches in pairs, so if there are 3, the first 2 will be matched and compared, but the 3rd won't.  If there are 4, the first 2 will be matched and the last 2 will be matched and compared.
 
**It matches in pairs, so if there are 3, the first 2 will be matched and compared, but the 3rd won't.  If there are 4, the first 2 will be matched and the last 2 will be matched and compared.
 
*Only mapped reads will be clipped
 
*Only mapped reads will be clipped
Line 97: Line 97:  
--stats        : Print some statistics on the overlaps.
 
--stats        : Print some statistics on the overlaps.
 
--overlapsOnly : Only output overlapping read pairs
 
--overlapsOnly : Only output overlapping read pairs
--excludeFlags : Skip records with any of the specified flags set, default 0x70C
+
--excludeFlags : Skip records with any of the specified flags set, default 0xF0C
 
                 --unmapped    : Mark records that would be completely clipped as unmapped
 
                 --unmapped    : Mark records that would be completely clipped as unmapped
 
  --noeof        : Do not expect an EOF block on a bam file.
 
  --noeof        : Do not expect an EOF block on a bam file.
Line 154: Line 154:     
=== Skip Records with any of the Specified Flags (<code>--excludeFlags</code>)===
 
=== Skip Records with any of the Specified Flags (<code>--excludeFlags</code>)===
Skip records with any of the specified flags set, default 0x70C
+
Skip records with any of the specified flags set, default 0xF0C
    
By default skips reads with any of the following flags set:
 
By default skips reads with any of the following flags set:
Line 162: Line 162:  
* fails QC checks
 
* fails QC checks
 
* duplicate
 
* duplicate
 
+
* supplementary
This parameter was added in version 1.0.10.
      
=== Mark entirely clipped reads as unmapped (<code>--unmapped</code>)===
 
=== Mark entirely clipped reads as unmapped (<code>--unmapped</code>)===

Navigation menu