FastQValidator

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Revision as of 22:30, 16 November 2009 by Goncalo (talk | contribs) (Created page with '== Status == The FastQ Validator is on our Todo List. == Valid FastQ File Requirements == A valid fastQ file should meet the following requirements: * A base sequence sho…')
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Status

The FastQ Validator is on our Todo List.

Valid FastQ File Requirements

A valid fastQ file should meet the following requirements:

  • A base sequence should have non-zero length.
  • A quality string should be present for every base sequence.
  • Paired quality and base sequences should be of the same length.
  • Valid quality values should all have ASCII codes > 32.
  • Valid bases should be ACTG or N, unless ambiguous bases are explicitly allowed by the application consuming the file.
  • Every entry in the file should have a unique identifier.