FastQValidator

Status

The FastQ Validator is on our Todo List.

Valid FastQ File Requirements

A valid fastQ file should meet the following requirements:

• A base sequence should have non-zero length.

• A quality string should be present for every base sequence.

• Paired quality and base sequences should be of the same length.

• Valid quality values should all have ASCII codes > 32.

• Valid bases should be ACTG or N, unless ambiguous bases are explicitly allowed by the application consuming the file.

• Every entry in the file should have a unique identifier.

• Reads should be of a minimum length; many mappers will get into trouble with very short reads.

• Base composition should be reported and tracked by position.