GotCloud: Alignment Pipeline

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Overview of Alignment Pipeline Steps

The Alignment/Mapping Pipeline takes FASTQ files and generates recalibrated BAM (Binary Sequence Alignment/Map format) files from them.

Running the GotCloud Alignment Pipeline

The alignment pipeline is run using the align option of the gotcloud script. This option calls align.pl found in the bin/ directory under the gotcloud installation.

Use the --conf parameter followed by the configuration file to specify the configuration to use for this run of the alignment pipeline.

You must specify the input list of FASTQs mapped to sample id to tell the alignment pipeline what files to process. You can do this by setting either:

• FASTQ_LIST in the configuration file
• --list on the command-line

You must specify an output directory to tell the alignment pipeline where to write its output by either setting:

• OUT_DIR in the configuration file
• --outdir on the command-line

Example of a Basic Alignment Command

gotcloud align --conf myAlignTest.conf --outdir ~/gotcloudOutput/align/

Running the Automated Test

The automated test runs the alignment pipeline on a small set of test data and checks that the results against expected results validating that GotCloud is installed correctly.

• Run alignment pipeline test:

gotcloud align --test OUTPUT_DIR 

where OUTPUT_DIR is the directory where you want to store the test results

If you see "Successfully ran the test case, congratulations!", then you are ready to align samples.

Input Data:

• Raw Sequence (FASTQ) files
• FASTQ List file mapping fastq pairs to sample (optional: Read Group information)
• Reference files
• (Optional) Configuration file to override default options

Raw Sequence (FASTQ) files

These are the FASTQ files that need to be mapped to BAM files.

These files are specified in the FASTQ List File.

FASTQ List File

This file specifies the FASTQ files that need to be processed. It maps the FASTQ pairs to the associated Sample ID. Optionally Read Group information for the FASTQ pairs can be specified. If the Read Group information is not specified, it is inferred.

This file is specified either via the command line parameter --list or via the configuration file setting FASTQ_LIST.

The command-line setting takes precedence over the configuration file setting.

The FASTQ list is a tab delimited file that starts with a header line. The columns may be in any order.

Following the header line, there is one line per single-end read and one line per paired-end read (only 1 line per pair).

Required Column Names:

• MERGE_NAME - base name for the resulting BAM file for the sample (used to group multiple fastqs or fastq pairs into a single BAM)
  • The SAMPLE column can be specified instead of MERGE_NAME. SAMPLE will be used for both the sample and the base name.
• FASTQ1 - name of the fastq or the first in the pair if paired-end. (Only 1 line per pair)

Optional Column Names:

• FASTQ2 - name of the 2nd fastq in paired-end reads. Specify '.' if the column exists, but this line is single-ended.
• RGID - Read Group ID for this entry
  • If this field is not specified, the first line of the fastq will be used to determine the RG.
    • If the first line does not match the expected format for determining RG, incrementing numbers per fastq file will be used.
• SAMPLE - Sample Name for this entry
  • If SAMPLE is not specified, MERGE_NAME will be used for the sample name
• LIBRARY - Library for this entry
  • If LIBRARY is not specified, the sample name will be used
• CENTER - Center Name for this entry
  • If CENTER is not specified, it will default to "unknown"
• PLATFORM - Platform for this entry
  • If PLATFORM is not specified, it will default to ILLUMINA

The RGID, SAMPLE, LIBRARY, CENTER, and PLATFORM are used to populate the Read Group information for this entry.

MERGE_NAME	FASTQ1	FASTQ2	RGID	SAMPLE	LIBRARY	CENTER	PLATFORM 
Sample1	fastq/S1/F1_R1.fastq.gz	fastq/S1/F1_R2.fastq.gz	RGID1	SampleID1	Lib1	UM	ILLUMINA 
Sample1	fastq/S1/F2_R1.fastq.gz	fastq/S1/F2_R2.fastq.gz	RGID1a	SampleID1	Lib1	UM	ILLUMINA 
Sample2	fastq/S2/F1_R1.fastq.gz	fastq/S2/F1_R2.fastq.gz	RGID2	SampleID2	Lib2	UM	ILLUMINA 
Sample2	fastq/S2/F2.fastq.gz	.	RGID2	SampleID2	Lib2	UM	ILLUMINA 

The --fastq_prefix/FASTQ_PREFIX setting can be used to specify a prefix to the FASTQ1/FASTQ2 file paths that should be applied before using the files.

Reference Files

The following Reference Files are required:

• Reference File fasta files
  • Files required: .fa, -bs.umfa, .GCContent, .amb, .ann, .bwt, .pac, .sa
    • If you don't have the -bs.umfa file, the software will try to create it in the same directory as the reference fasta.
    • .GCContent can be generated using qplot, see: QPLOT: Input Files: --gccontent and name the resulting file as .fa.GCcontent
    • Use bin/bwa index ref.fa if you need to generate the bwa reference files (.amb, .ann, .bwt, .pac, .sa)
  • Configuration Name: REF - specify the ref.fa/ref.fa.gz name
• DBSNP File
  • VCF file containing dbsnp variants
  • Configuration Name: DBSNP_VCF
• HapMap3 VCF
  • VCF file containing HM3 variants
  • Configuration Name: HM3_VCF

Configuration File

Configuration file contains the run-time options including the software binaries and command line arguments. A default configuration file is automatically loaded. Users may specify their own configuration file specifying just the values different than the defaults. The configuration file is not required if there are no values to override.

Comments begin with a #

Format: KEY = value

Where KEY is the item being set and value is its new value

See Command-Line Options for values that can be set either via command line or via configuration.

Note: Command-line options take priority over configuration file settings

Required Settings

See Reference Files for the required reference file settings.

See Sequence Index File for how to set the index file either via command line options or via configuration.

Turning Off Optional Steps

Quality Control steps can be disabled.

To Disable QPLOT, remove qplot from the PER_MERGE_STEPS configuration by setting:

PER_MERGE_STEPS = verifyBamID index recab

To Disable VerifyBamID, remove qplot from the PER_MERGE_STEPS configuration by setting:

PER_MERGE_STEPS = qplot index recab

Optional Configurable Settings

You may want to adjust the amount of memory/threads that are used:

There are additional configurable settings, but these are the ones most likely to be adjusted.

• BWA_THREADS = -t N
  • Fill in the N with the number of threads you want BWA to run with, default is 1
• SORT_MAX_MEM = 2000000000
  • Maximum amount of memory used by samtools sort after running bwa
• BATCH_TYPE = type
  • Tells GotCloud to use "type" for sending jobs to the cluster
  • "type" can be mosix, slurm, slurmi, pbs, sge, sgei
• BATCH_OPTS = options
  • replace "options" with the options you want to pass to your cluster type
  • For example: BATCH_OPTS = -j36,37,38,39,40,41,45,46,47,48,49
    • Specifies which client nodes mosix should send jobs to.

Running the Alignment Pipeline

Command-Line Options

• help - print usage
• test OUTPUT_DIR - run the test example placing the output in a user specified OUTPUT_DIR. No other options are required.
• outdir OUTPUT_DIR - directory for the output
  • May also be specified via OUT_DIR in the configuration file
  • Required to be set either via command-line or configuration
• conf CONFIG_FILE - configuration file
• list FASTQ_LIST_FILE_NAME - name of the fastq list file
  • May also be specified via FASTQ_LIST in the configuration file
  • Required to be set either via command-line or configuration
• ref_dir REFERENCE_DIR - value to set config key REF_DIR to, overriding other values, REF_DIR can then be used inside config files.
  • May also be specified via REF_DIR in the configuration file
• ref_prefix REFERENCE_DIR - path to prepend to non-absolute REF paths.
  • May also be specified via REF_PREFIX in the configuration file
• fastq_prefix FASTQ_PATH - prefix path to the fastq files specified in the FASTQ_LIST
  • May also be specified via FASTQ_PREFIX in the configuration file
• base_prefix BASE_PATH - prefix path to the prepend to fastq/ref files without absolute paths
  • May also be specified via BASE_PREFIX in the configuration file
• keepTmp - Do not remove the temporary files (removed by default)
  • May also be specified via KEEP_TMP in the configuration file
• keepLog - Do not remove the intermediate log files (removed by default)
  • May also be specified via KEEP_LOG in the configuration file
• numjobs N - Replace N with the number of samples that should be processed in parallel
• threads N - Replace N with the number of targets in each makefile that should be run in parallel
• dryrun - Create the Makefile, but do not run it
• maxlocaljobs N - Replace N with the maximum number of jobs that can be run locally (no batchtype specified). Default is 10.
• batchtype TYPE - Tells GotCloud the specified batch type to send jobs to the client nodes
  • May also be specified via BATCH_TYPE in the configuration file
  • Can be: mosix, slurm, slurmi, pbs, sge, sgei
• batchopts OPTS - Tells GotCloud the options to pass onto the batch system
  • May also be specified via BATCH_OPTS in the configuration file
• noPhoneHome - disable the phone home logic
• gotcloudroot DIR - Specifies an alternate path to other gotcloud files rather than using the path to the gotcloud/align.pl.

Note: Command-line options take priority over configuration file settings

Running the Alignment Pipeline

Run gotcloud align with the appropriate command-line parameters.

Example:

gotcloud align --conf config.txt --outdir output 

This step generates 1 Makefile per sample in the output/Makefiles/ directory and then automatically runs them. The Makefiles contain all of the information to run each sample.

If you only want to generate the makefiles and not run them, use the --dryrun option. It will generate the Makefiles and print instructions for running the Makefiles.

Each Makefile is independent and can be run in parallel and across a cloud.

On success, you will see:

Processing finished in nn secs with no errors reported 

and should see the following subdirectories under the user specified output directory:

• bams/
• Makefiles/
• QCFiles/ (if all quality control is not disabled)
• tmp/

You should see a .OK for each Sample in the index file.

If you do not see these .OK files, then your Alignment Pipeline failed.

On success, the bams/ directory contains the final BAMs and bais.

If processing fails part way through, you can pick up where you left off by rerunning gotcloud or the make command.