GotCloud: Genetic Reference and Resource Files

Installing Genetic Reference and Resource Files

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Get the Resource Files The GotCloud Aligner and Umake makes use of various reference and other genetic resource files. You are free to use your own files, of course, but we also are making the files we use available.

#  The easiest way to get the data:
cd /tmp
wget ftp://share.sph.umich.edu/gotcloud/hs37-db132.tar.gz

#  Another way:
cd /tmp
ftp share.sph.umich.edu
Connected to share.sph.umich.edu.
220 (vsFTPd 2.3.5)
Name (share.sph.umich.edu:tpg): anonymous
230 Login successful.
Remote system type is UNIX.
Using binary mode to transfer files.
ftp> prompt
Interactive mode off.
ftp> cd gotcloud
250 Directory successfully changed.
ftp> mget hs37-db132.tar.gz
ftp> quit
221 Goodbye.

Install the Resource Files

Choose a destination for these files and install them as shown below (we'll assume you will use /usr/local/gotcloud.ref).

mkdir -p /usr/local/gotcloud.ref    # Where you want the files installed
cd /usr/local/gotcloud.ref
tar xzvf hs37-db132.tar.gz
 ref/
 ref/hs37d5.fa.fai
 ref/metabochip.batch2.broken.b37.chr2.plink.MAF01.bed
 ref/hs37d5-bs.umfa
 ref/metabochip.batch2.broken.b37.chr2.plink.MAF01.fam
 ref/dbsnp_132.b37.vcf.gz.tbi
 ref/dbsnp_132.UCSC.coordinates.tbl
   [lines deleted]
rm -f hs37-db132.tar.gz

Note this path as you will need to set the variable REF_DIR in the configuration file or options gen_biopipeline.pl and umake.pl.