Changes

[[Media: WrongBarCoding.pdf | Example of qplot identifying the effect of ignoring bar-coding]]

[[Media: WrongBarCoding.pdf | Example of qplot identifying the effect of ignoring bar-coding]]

By checking "Empirical phred score by cycle" (top right graph on the first page), we notice the empirical qualities in the first several cycle are abnormally low. This question leads us hypnotize the first several bases have different properties. Further investigation revealed that this sequencing was done using bar-coded DNA samples, but the analysis did not properly de-multiplexing to each sample.

By checking "Empirical phred score by cycle" (top right graph on the first page), we noticed the empirical qualities in the first several cycle are abnormally low. This phenomenon leads us to hypothesize the first several bases have different properties. Further investigation confirmed that this sequencing was done using bar-coded DNA samples, but the analysis did not properly de-multiplexing to each sample.

= Contact =

= Contact =

Questions and requests should be sent to Bingshan Li ([mailto:bingshan@umich.edu bingshan@umich.edu])

Questions and requests should be sent to Bingshan Li ([mailto:bingshan@umich.edu bingshan@umich.edu])