RAREMETALWORKER METHOD

Brief Introduction

RAREMETALWORKER(RMW) generates single variant association results from score test, together with summary statistics and covariance matrices of the score statistics.

In the following sections, we will go through the methods behind RWM including statistic model, handling sample relatedness, and definitions of statistics in the output.

Modeling Relatedness

we use a variance component model to handle familial relationships. In a sample of n individuals, we model the observed phenotype vector (y) as a sum of covariate effects (specified by a design matrix X and a vector of covariate effects β), additive genetic effects (modeled in vector g) and non-shared environmental effects (modeled in vector ε). Thus the null model is:

$\mathbf {y} =\mathbf {X} \beta +\mathbf {g} +{\boldsymbol {\varepsilon }}$

We assume that genetic effects are normally distributed, with mean $\mathbf {0}$ and covariance $\mathbf {K} \sigma _{g}^{2}$ where the matrix K summarizes kinship coefficients between sampled individuals and $\sigma _{g}^{2}$ is a positive scalar describing the genetic contribution to the overall variance. We assume that non-shared environmental effects are normally distributed with mean $\mathbf {0}$ and covariance $\mathbf {I} \sigma _{e}^{2}$, where $\mathbf {I}$ is the identity matrix.