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;Do you notice a difference between this index and yours?
 
;Do you notice a difference between this index and yours?
 
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<li>Answer:</li>
 
<li>Answer:</li>
 
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<li>It doesn't have a full path to the BAM file, while your index has /home/...</li>
 
<li>It doesn't have a full path to the BAM file, while your index has /home/...</li>
 
[[File:Bamindex1.png|300px]]
 
[[File:Bamindex1.png|300px]]
<li>That's ok, <code>gotcloud.conf</code> contains the path to those BAMs</li>
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<li>That's ok, we will use the <code>--base_prefix ${SS}</code> command-line option to prefix the BAM paths</li>
[[File:BamindexConf.png|300px]]
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<li>Alternatively, we could have set BAM_INDEX in <code>gotcloud.conf</code> to the path to the BAMs
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<pre>BAM_INDEX = /home/username/seqshop/example</pre> </li>
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<li>NOTE: the conf file can't interpret ${SS} environment variables or '~', so you would have to specify the full path</li>
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<li>We just used the command-line option for this tutorial since this path will vary by user.</li>
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