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| * Understand how to visualize the association output from EPACTS | | * Understand how to visualize the association output from EPACTS |
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− | == Setup in person at the SeqShop Workshop == | + | == Setup == |
| ''This section is specifically for the SeqShop Workshop computers.'' | | ''This section is specifically for the SeqShop Workshop computers.'' |
− | <div class="mw-collapsible mw-collapsed" style="width:600px"> | + | <div class="mw-collapsible" style="width:600px"> |
| ''If you are not running during the SeqShop Workshop, please skip this section.'' | | ''If you are not running during the SeqShop Workshop, please skip this section.'' |
| <div class="mw-collapsible-content"> | | <div class="mw-collapsible-content"> |
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− | <div class="mw-collapsible mw-collapsed" style="width:600px">
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− | ''If you are not already logged in, please expand this section.''
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− | <div class="mw-collapsible-content">
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| {{SeqShopLogin}} | | {{SeqShopLogin}} |
| </div> | | </div> |
| </div> | | </div> |
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− | === Setup your run environment=== | + | === Prerequisite Tutorials === |
− | This is the same setup you did for the previous tutorial, but you need to redo it each time you log in.
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− | | |
− | This will setup some environment variables to point you to
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− | * [[GotCloud]] program
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− | * Tutorial input files
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− | * Setup an output directory
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− | ** It will leave your output directory from the previous tutorial in tact.
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− | source /net/seqshop-server/home/mktrost/seqshop/setup.txt
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− | * You won't see any output after running <code>source</code>
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− | ** It silently sets up your environment
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− | ** If you want to view the detail of the setup, type
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− | less /net/seqshop-server/home/mktrost/seqshop/setup.txt
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− | and press 'q' to finish.
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− | | |
− | <div class="mw-collapsible mw-collapsed" style="width:200px">
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− | View setup.txt
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− | <div class="mw-collapsible-content" style="width:800px">
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− | export GC=/net/seqshop-server/home/mktrost/seqshop/gotcloud
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− | export SS=/net/seqshop-server/home/mktrost/seqshop/example
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− | export EPACTS=/net/seqshop-server/home/mktrost/seqshop/epacts
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− | export OUT=~/out
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− | mkdir -p ${OUT}
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− | </div>
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− | </div>
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− | </div>
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− | </div>
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− | | |
− | == Setup when running on your own outside of the SeqShop Workshop ==
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− | ''This section is specifically for running on your own outside of the SeqShop Workshop.''
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− | <div class="mw-collapsible" style="width:600px">
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− | ''If you are running during the SeqShop Workshop, please skip this section.''
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− | <div class="mw-collapsible-content">
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| This tutorial builds on the alignment & snpcall tutorials, if you have not already, please first run those tutorials: [[SeqShop:_Sequence_Mapping_and_Assembly_Practical|Alignment Tutorial]] & [[SeqShop: Variant Calling and Filtering for SNPs Practical|SNP Calling Tutorial]] | | This tutorial builds on the alignment & snpcall tutorials, if you have not already, please first run those tutorials: [[SeqShop:_Sequence_Mapping_and_Assembly_Practical|Alignment Tutorial]] & [[SeqShop: Variant Calling and Filtering for SNPs Practical|SNP Calling Tutorial]] |
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| cd ~/seqshop/ | | cd ~/seqshop/ |
| * download, decompress, and build the version of epacts that was tested with this tutorial: | | * download, decompress, and build the version of epacts that was tested with this tutorial: |
− | wget http://www.sph.umich.edu/csg/kang/epacts/download/EPACTS-3.2.6.tar.gz | + | wget http://csg.sph.umich.edu/kang/epacts/download/EPACTS-3.2.6.tar.gz |
| tar xvf EPACTS-3.2.6.tar.gz | | tar xvf EPACTS-3.2.6.tar.gz |
| cd EPACTS-3.2.6 | | cd EPACTS-3.2.6 |