Changes

From Genome Analysis Wiki
Jump to navigationJump to search
62 bytes added ,  02:03, 13 December 2010
no edit summary
Line 50: Line 50:  
   Go to http://genome.ucsc.edu/ ►► Click "table" ►► Specify the fields required (clade: mammal, genome:human etc.) ►► In "track" filed, select "UCSC gene" ►► get output gene file
 
   Go to http://genome.ucsc.edu/ ►► Click "table" ►► Specify the fields required (clade: mammal, genome:human etc.) ►► In "track" filed, select "UCSC gene" ►► get output gene file
 
   
 
   
  1. Gene file used should be of [http://genome.ucsc.edu/FAQ/FAQformat#format9 GenePred table format]. The following 10 fields are required and must be of the same order as shown below:
+
  1. Gene file used should be of [http://genome.ucsc.edu/FAQ/FAQformat#format9 GenePred table format]. The following 11 fields are required and must be of the same order as shown below:
 
     string  name;              "Name of gene"
 
     string  name;              "Name of gene"
 
     string  chrom;              "Chromosome name"
 
     string  chrom;              "Chromosome name"
Line 61: Line 61:  
     uint[exonCount] exonStarts; "Exon start positions"
 
     uint[exonCount] exonStarts; "Exon start positions"
 
     uint[exonCount] exonEnds;  "Exon end positions"
 
     uint[exonCount] exonEnds;  "Exon end positions"
 +
    string  symbol;            "Standard gene symbol"
 +
Note:
 
  2. If gene file assumes an [http://genome.ucsc.edu/FAQ/FAQformat#format9 extended GenePred format], there will be an exctra "exonframe" field. Please refer to [https://lists.soe.ucsc.edu/pipermail/genome/2006-November/012218.html here] for the definition of "exonframe". For some genes, due to translational frame shifts or other  
 
  2. If gene file assumes an [http://genome.ucsc.edu/FAQ/FAQformat#format9 extended GenePred format], there will be an exctra "exonframe" field. Please refer to [https://lists.soe.ucsc.edu/pipermail/genome/2006-November/012218.html here] for the definition of "exonframe". For some genes, due to translational frame shifts or other  
 
     reasons, the exonframe might not match what one would compute using mod 3 in counting codons. In such cases, the program will report a warning massage that "number of base pairs between code start and code end is
 
     reasons, the exonframe might not match what one would compute using mod 3 in counting codons. In such cases, the program will report a warning massage that "number of base pairs between code start and code end is
76

edits

Navigation menu