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, 17:49, 19 October 2014
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| The alignment pipeline is run using the <code>align</code> option of the <code>gotcloud</code> script. This option calls <code>align.pl</code> found in the <code>bin/</code> directory under the <code>gotcloud</code> installation. | | The alignment pipeline is run using the <code>align</code> option of the <code>gotcloud</code> script. This option calls <code>align.pl</code> found in the <code>bin/</code> directory under the <code>gotcloud</code> installation. |
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− | You must specify the <code>--conf</code> parameter followed by the configuration file to be used for this run of the alignment pipeline.
| + | Use the <code>--conf</code> parameter followed by the configuration file to specify the configuration to use for this run of the alignment pipeline. |
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− | If your configuration file does not contain an output directory, you must specify <code>--outdir</code> on the command-line to tell the alignment pipeline where to write its output.
| + | You must specify the input list of FASTQs mapped to sample id to tell the alignment pipeline what files to process. You can do this by setting either: |
| + | * <code>FASTQ_LIST</code> in the configuration file |
| + | * <code>--list</code> on the command-line |
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| + | You must specify an output directory to tell the alignment pipeline where to write its output by either setting: |
| + | * <code>OUT_DIR</code> in the configuration file |
| + | * <code>--outdir</code> on the command-line |
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| '''Example of a Basic Alignment Command''' | | '''Example of a Basic Alignment Command''' |
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| If you see "Successfully ran the test case, congratulations!", then you are ready to align samples. | | If you see "Successfully ran the test case, congratulations!", then you are ready to align samples. |
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| == Input Data:== | | == Input Data:== |