From Genome Analysis Wiki
Jump to navigationJump to search
10 bytes added
, 10:11, 23 February 2015
Line 139: |
Line 139: |
| or | | or |
| --qcfail_keep | | --qcfail_keep |
| + | |
| | | |
| *Records to process | | *Records to process |
Line 149: |
Line 150: |
| | | |
| '''NOTE''' In order for this to work, the lane info has to be encoded in the read name such that the lane number is the second field with the delimiter ":". | | '''NOTE''' In order for this to work, the lane info has to be encoded in the read name such that the lane number is the second field with the delimiter ":". |
| + | |
| | | |
| * Read group to process : | | * Read group to process : |
| | | |
− | Read group option can restrict qplot to process a subset of reads. For example, if BAM contain the following @RG tags:
| + | The read group option can restrict qplot to process a subset of reads. For example, if the BAM contains the following @RG tags: |
| | | |
| @RG ID:UM0348_1:1 PL:ILLUMINA LB:M5390 SM:M5390 CN:UM | | @RG ID:UM0348_1:1 PL:ILLUMINA LB:M5390 SM:M5390 CN:UM |
Line 163: |
Line 165: |
| @RG ID:UM0360_4:1 PL:ILLUMINA LB:M5390 SM:M5390 CN:UM | | @RG ID:UM0360_4:1 PL:ILLUMINA LB:M5390 SM:M5390 CN:UM |
| | | |
− | If specify nothing or not using "--readGroup", QPLOT by default will process all reads;
| + | QPLOT will by default (without specifying --readgroup) process all reads. |
− | If specify "--readGroup UM0348", then only read group UM0348_1, UM_0348_2, UM_0348_3, UM_0348_4 will be processed; | + | |
− | If specify "--readGroup UM0348_1", then only one read group UM0348_1 will be processed. | + | If you specify "--readGroup UM0348", then only read groups UM0348_1, UM_0348_2, UM_0348_3, UM_0348_4 will be processed. |
| + | |
| + | If you specify "--readGroup UM0348_1", then only one read group, UM0348_1, will be processed. |
| + | |
| | | |
| * Input file options : | | * Input file options : |
Line 176: |
Line 181: |
| | | |
| Qplot will exclude reads with lower mapping qualities than the user specified parameter, <code>--minMapQuality</code>. By default, mapped reads with all mapping quality will be included in the analysis. | | Qplot will exclude reads with lower mapping qualities than the user specified parameter, <code>--minMapQuality</code>. By default, mapped reads with all mapping quality will be included in the analysis. |
| + | |
| | | |
| *Region list | | *Region list |