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→Parameters
= Parameters =
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The file does not need to be strictly sorted by read name. The only requirement is that matching read names are next to each other.
=== Split into RG Specific FASTQs (<code>--splitRG</code>) ===
Create RG specific FASTQ files.
Cannot be specified with firstOut/secondOut/unpairedOut since there will be a different filename for each RG.
Cannot write to stdout when <code>--splitRG</code> is specified.
Output filenames will be <outBase>.<RG>_1.fastq, <outBase>.<RG>_2.fastq, and <outBase>.<RG>.fastq. A fastq list file <outBase>.list will be created containing MERGE_NAME (the RG tag's SM value or outBase if the value is empty), fastq 1, fastq 2 (or . if it is a single ended fastq), and the RG tag string.
=== Use the Base Quality from the Specified Tag (<code>--qualField</code>) ===
By default, the quality field is used for the Base Qualities in the FASTQ file. Specify <code>--qualField <tagName></code> to use the base qualities from the specified tag instead of the quality field.
=== Generate 1 Paired-End Output File (<code>--merge</code>) ===
Specifying <code>--noReverseComp</code> would result in a FASTQ sequence of ACCGTG
=== Only Convert the Specified Region (<code>--region</code>) ===
Only convert reads containing the specified region/nucleotide.
Position formatted as: chr:pos:base
pos (0-based) & base are optional.
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