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Besides the example of analyzing RNA-seq data as shown in our manuscript,
Besides the example of analyzing RNA-seq data as shown in our manuscript,
here we demonstrate two additional scenarios in which qplot can help identify problems after obtaining sequencing data.
here we demonstrate two additional scenarios in which qplot can help identify problems after obtaining sequencing data.
* Base quality distributed abnormally
* Base quality distributed abnormally
In this particular example, '33' was incorrectly added to all base qualities.
In this particular example, '33' was incorrectly added to all base qualities.
When such data used in variant calling, we may increase false positive SNP variants.
When such data used in variant calling, we may increase false positive SNP variants.
* Bar-coded samples
* Bar-coded samples
