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Either pipeline can be run on a single machine or on a cluster of machines.
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= Genomes on the Cloud (GotCloud)=
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To handle the increasing volume of next generation sequencing and genotyping data, we created and developed software pipelines called '''Genomes on the Cloud (GotCloud)''' for:
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*Mapping
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*Variant Calling
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Key Features:
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*Scalable to tens of thousands of samples
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*Robust against unexpected system failure using GNU make
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*Massively parallel, can run hundreds of jobs
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*Easy to use - Automates series of configurable steps
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*Available on Amazon Web Services (AWS) Elastic Compute Cloud (EC2)
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*Run on local machines/clusters
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*Available via Debian Packages
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The following describes the use of this software with the Amazon Web Services (https://aws.amazon.com/),
 
The following describes the use of this software with the Amazon Web Services (https://aws.amazon.com/),
but you can just as easily use it on your own machine(s) by just installing it.
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but you can just as easily use the pipelines on your own machine(s) by just installing them.
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== AWS Specific Setup ==
 
'''Preparation in AWS'''
 
'''Preparation in AWS'''
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* Notes on sequence data preparation in [[Amazon Storage|Amazon Storage]].
 
* Notes on sequence data preparation in [[Amazon Storage|Amazon Storage]].
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'''Install the Software'''
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'''Resources / Cost'''
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* [[AWS Rresources|AWS Resources]]
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'''Development Notes'''
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* [[Creating an AMI on EC2]]
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* [[Mount S3 Volume]]
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== General Usage ==
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'''Install the Software (for AWS or a local machine)'''
    
* Installing the software as a [[Pipeline Debian Package|debian package]]
 
* Installing the software as a [[Pipeline Debian Package|debian package]]
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* [[Alignment Pipeline]]  
 
* [[Alignment Pipeline]]  
 
* [[Variant Calling Pipeline (UMAKE)]]
 
* [[Variant Calling Pipeline (UMAKE)]]
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'''Resources / Cost'''
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* [[AWS Rresources|AWS Resources]]
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'''Development Notes'''
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* [[Creating an AMI on EC2]]
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* [[Mount S3 Volume]]
 

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