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See [[#Sequence Index File|Sequence Index File]] for how to set the index file either via command line options or via configuration.
 
See [[#Sequence Index File|Sequence Index File]] for how to set the index file either via command line options or via configuration.
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See [[#Specifying the Output Directory|Specifying the Output Directory]] for how to set the output directory.
      
==== Turning Off Optional Steps====
 
==== Turning Off Optional Steps====
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* out_dir OUTPUT_DIR - directory for the output
 
* out_dir OUTPUT_DIR - directory for the output
 
** May also be specified via OUT_DIR in the configuration file
 
** May also be specified via OUT_DIR in the configuration file
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** Required to be set either via command-line or configuration
 
* conf CONFIG_FILE - configuration file
 
* conf CONFIG_FILE - configuration file
 
* index_file INDEX_FILE_NAME  - name of the index file
 
* index_file INDEX_FILE_NAME  - name of the index file
 
** May also be specified via INDEX_FILE in the configuration file
 
** May also be specified via INDEX_FILE in the configuration file
* ref_dir REFERENCE_DIR - value to set config key REF_DIR to, overriding other values, REF_DIR can then be used inside config files.
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** Required to be set either via command-line or configuration* ref_dir REFERENCE_DIR - value to set config key REF_DIR to, overriding other values, REF_DIR can then be used inside config files.
 
** May also be specified via REF_DIR in the configuration file
 
** May also be specified via REF_DIR in the configuration file
 
* fastq FASTQ_PATH - prefix path to the fastq files specified in the INDEX_FILE
 
* fastq FASTQ_PATH - prefix path to the fastq files specified in the INDEX_FILE

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