Changes

We will start with a set of short sequence reads and associated base quality scores (stored in a fastq file), find the most likely genomic location for each read (producing a BAM file), generate an initial list of polymorphic sites and genotypes (stored in a VCF file) and use haplotype information to refine these genotypes (resulting in an updated VCF file).

We will start with a set of short sequence reads and associated base quality scores (stored in a fastq file), find the most likely genomic location for each read (producing a BAM file), generate an initial list of polymorphic sites and genotypes (stored in a VCF file) and use haplotype information to refine these genotypes (resulting in an updated VCF file).

'''Note:''' if you are interested in detecting '''de novo mutations''', please try the other program [http://genome.sph.umich.edu/wiki/Polymutt Polymutt] we developed.  

'''Note:''' if you are interesting in detecting '''de novo mutations''', or are working on a small number of families with high coverage data (e.g. exome sequencing),

please try the other program [http://genome.sph.umich.edu/wiki/Polymutt Polymutt] we developed.  

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