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Note this path as you will need to set the variable '''REF_DIR''' in the configuration file or options
 
Note this path as you will need to set the variable '''REF_DIR''' in the configuration file or options
 
'''gen_biopipeline.pl''' and '''umake.pl'''.
 
'''gen_biopipeline.pl''' and '''umake.pl'''.
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= Using Your own Reference Files =
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== Human Reference ==
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=== Generating GCContent File ===
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To generate the GC content file, run qplot:
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GOTCLOUD_DIR/bin/qplot --reference reference.fa --winsize windowSize --create_gc reference.gc
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* Replace reference.fa with the name of your human reference fasta file.
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* Replace windowSize with your desired window size, or leave out --winsize to use the default.
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http://genome.sph.umich.edu/wiki/QPLOT#Input_files

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