From Genome Analysis Wiki
Jump to navigationJump to search
453 bytes added
, 12:31, 5 April 2013
Line 70: |
Line 70: |
| Note this path as you will need to set the variable '''REF_DIR''' in the configuration file or options | | Note this path as you will need to set the variable '''REF_DIR''' in the configuration file or options |
| '''gen_biopipeline.pl''' and '''umake.pl'''. | | '''gen_biopipeline.pl''' and '''umake.pl'''. |
| + | |
| + | |
| + | = Using Your own Reference Files = |
| + | |
| + | == Human Reference == |
| + | |
| + | === Generating GCContent File === |
| + | To generate the GC content file, run qplot: |
| + | GOTCLOUD_DIR/bin/qplot --reference reference.fa --winsize windowSize --create_gc reference.gc |
| + | * Replace reference.fa with the name of your human reference fasta file. |
| + | * Replace windowSize with your desired window size, or leave out --winsize to use the default. |
| + | http://genome.sph.umich.edu/wiki/QPLOT#Input_files |