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RAREMETAL Documentation

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'''rareMETAL''' is a tool for gene-based meta-analysis, based upon summary statistics generated from individual data using [http[Category://genome.sph.umich.edu/wiki/Rare-Metal-Worker '''Rare Metal Worker'''RAREMETAL]. Another implementation of the same methods in R-package can be found [http://genome.sph.umich.edu/wiki/RareMETALS '''RareMetals'''].== Useful Wiki Pages ==
If you have any questions, please contact* Git hub page: sfengsph at umich dot eduhttps://github.com/statgen/Raremetal
* The [[RAREMETAL_Change_Log | Change Log]]
* The [[RAREMETAL_DOWNLOAD_%26_BUILD | DOWNLOAD page]]
== Useful Wiki Pages ==* The [[Tutorial:_RAREMETAL|RAREMETAL Quick Start Tutorial]]* The [[RAREMETAL METHOD]]
There are a few pages in this Wiki that may be useful to rareMETAL users. Here are links to key pages:* The [[RAREMETAL FAQ]]
* The [[rareMETAL|rareMETAL Home PageRAREMETAL Command Reference]]
* The [[Tutorial:_RareMETALRAREMETALWORKER|rareMETAL Quick Start TutorialRAREMETALWORKER documentation]]
* The [[rareMETAL FAQ]http://genome.sph.umich.edu/wiki/Rvtests '''rvtests''']tool for rare-variant association analysis can also generate output compatible with RAREMETAL.
* The [[rareMETAL Command Reference]]== Key Features Brief Description =='''rareMETAL''' has the following features:* '''rareMETALRAREMETAL''' performs geneis a computationally efficient tool for meta-based analysis of rare variants using sequencing or regiongenotyping array data. It takes summary statistics and LD matrices generated by [[Rare-based meta analysis using Burden tests with the following methods: CMC_counts, MadsenMetal-Browning, SKAT, and Variable Threshold. * Worker|'''rareMETALRAREMETALWORKER''' performs single variant metal-analysis by default]] or [http://genome.sph. * '''rareMETAL''' allows customized groups of variants to be testedumich.* edu/wiki/Rvtests '''rareMETALrvtests''' allows conditional analysis to be performed in ], handles related and unrelated individuals, and supports both gene-level meta-analysis single variant and single variants burden meta-analysis.* '''rareMETAL''' generated QQ plots and manhattan It generates high quality plots by defaultand has options that allow users to build reports at different levels.
== Brief Description =='''RAREMETAL''' is developed by Shuang Feng, Dajiang Liu and Gonçalo Abecasis. A R-package written by Dajiang Liu using the same methodology is [[RareMetals|'''available''']].
rareMETAL is a tool for == Key Features =='''RAREMETAL''' has the following features:* Performs gene-based or region-based metaanalysis using Burden tests with the following methods: CMC_counts, Madsen-Browning, SKAT, and Variable Threshold. * Performs single variant metal-analysis rare variants from genotype arrays and sequencingby default. rareMETAL can combine summary statistics * Allows customized groups of individual studies generated by [[Rare-Metal-Worker|rareMetalWorker]]variants to be tested. It provides a convenient approach for * Allows conditional analysis to be performed in both single variant and gene-level meta-analysis of rare and single variants from various studies, when jointmeta-analysis of raw data from these studies are restricted.* Generate QQ plots and manhattan plots by default.
== Approach ==
The key idea behind meta-analysis with rareMETAL RAREMETAL is that various gene-level test statistics can be reconstructed from single variant score statistics and that, when the linkage disequilibrium relationships between variants are known, the distribution of these gene-level statistics can be derived and used to evaluate signifi-cance. Single variant statistics are calculated using the Cochran-Mantel-Haenszel method. The main formulae are tabulated Our method has been published in the following[http{| border="1" cellpadding="5" cellspacing="0" align="center"|+'''Formulae for rareMETAL'''! scope="col" width="120pt" | Test! scope="col" width="50pt" | Statistics! scope="col" width="225pt" | Null Distribution|-| Single Variant || <math>T=\sum_{i=1}^n {U_i}\bigg/\sqrt{\sum_{i=1}^n{V_i}}</math> || <math>T\sim\mathbf{N}(0,1)<www.nature.com/math>|-| un-weighted Burden || <math>T_b=\sum_{i=1}^n{\mathbf{U_i}}\Bigng/\sqrt{\sum_{i=1}^n{\mathbf{V_i}}}<journal/math> || <math>T_b\sim\mathbf{N}(0,1)<v46/math>|-| Weighted Burden || <math>T_{wb}=\mathbf{w^T}\sum_{i=1}^n{\mathbf{U_i}}\biggn2/\sqrt{\mathbf{w^T}\left(\sum_{i=1}^n{\mathbf{V_i}}\right)\mathbf{w}}<abs/math> || <math>T_{wb}\sim\mathbf{N}(0,1)</math>|-style="heightng.2852.html '''Liu et. al'''] in Nature Genetics. Please go to [http: 50pt;"| VT || <math>T_{VT}=\max(T_{b\left(f_1\right)},T_{b\left(f_2\right)},\dots,T_{b\left(f_m\right)}),\text{ where}</math><math>T_{b\left(f_i\right)}=\boldsymbol{\nu}_{f_i}^\mathbf{T}\sum_{i=1}^n{\mathbf{U_i}}\bigg/\sqrt{\boldsymbol{\nu}_{f_i}^\mathbf{T}\left(\sum_{i=1}^n{\mathbf{V_i}}\right)\boldsymbol{\nu}_{f_i}}\text{, where}</math> <math> \boldsymbol{\nu}_{f_i}\text{ is a indicator vectorgenome.sph.umich.} <edu/math> ||<math> \left(T_{b\left(f_1\right)},T_{b\left(f_2\right)},\dots,T_{b\left(f_m\right)}\right)<wiki/math><math>\sim\mathbf{MVN}\left(\mathbf{0},\boldsymbol{\Phi}^\mathbf{T}\sum_{i=1}^n{\mathbf{V_i}}\boldsymbol{\Phi}\right)</math>|-| SKAT || <math>\mathbf{Q}=\left(\sum_{i=1}^n{\mathbf{U_i^T}}\right) \mathbf{W}\left(\sum_{i=1}^n{\mathbf{U_i}}\right)</math> ||<math>\mathbf{Q}\sim\sum_{i=1}^m{\lambda_i\chi_{1,i}^2},\text{ where}</math> <math>\left(\lambda_1,\lambda_2,\dots,\lambda_m\right)\text{ are eigen values of}</math><math>\left(\sum_{i=1}^n{\mathbf{V_i}}\right)^\frac{1}{2}\mathbf{W}\left(\sum_{i=1}^n{\mathbf{V_i}}\right)^\frac{1}{2}</math>|}RAREMETAL_method '''method'''] for details.
== Download and Installation ==
* University of Michigan CSG users can go to the following:
/net/fantasia/home/sfengsph/code/Rare-Metal/raremetal/bin/raremetal
=== Where to Download ===* The software package for We have tested compilation using our source code on several platforms including Linux , and Mac (source code included) can be downloaded here: [[Media:raremetal.0.0.2OS X.tgz|software package download]]
=== How For source code and executables together with instructions of building from source, please go to Compile ===* Save it to your local path [[RAREMETAL_DOWNLOAD_%26_BUILD |'''DOWNLOAD source and decompress using the following command: tar xvzf raremetal.0.0.1.tgz* Go to raremetal_0executables''']].0.1/raremetal/src and type the following command to compile: make
=== How to Execute ===* Go to raremetal_0.0.1/raremetal/bin and use the following: ./raremetal* For example usagequestions about compilation, please refer go to [[http://genome.sph.umich.edu/wiki/Rare-Metal#Example_Usage example command linesRAREMETAL_FAQ | '''FAQ''']].
== Basic Usage Instructions ==
raeMETAL '''RAREMETAL''' is a command line tool. It is typically run from a Linux or Unix prompt by invoking the command <code>raremetal</code>. An example In the following are descriptions of basic usage for meta analysis. A detailed [[Tutorial:_RareMETAL|'''TUTORIAL''']] with toy data are also available. ==== Prepare Input Files===='''RAREMETAL''' requires the following basic input files: summary statistics and covariance matrices of score statistics generated by '''RAREMETALWORKER''' or [http://genome.sph.umich.edu/wiki/Rvtests '''rvtests'''], a file with list of studies to be included and a group file if gene-level meta-analysis is included at expected.   =====Summary Statistics=====Files containing summary statistics and LD matrices generated by '''RAREMETALWORKER''' should be compressed and [http://samtools.sourceforge.net/tabix.shtml '''tabix'''] indexed using the bottom of this pagefollowing commands (Note in RAREMETALWORKER, if --zip is specified, these .gz and .tbi files will be automatically generated):  bgzip study1.singlevar.score.txt tabix -s 1 -b 2 -e 2 -c "#" study1.singlevar.score.txt.gz bgzip study1.singlevar.cov.txt tabix -s 1 -b 2 -e 2 -c "#" study1.singlevar.cov.txt.gz
A detailed Files containing summary statistics and LD matrices generated by '''rvtests''' should be compressed and [[Tutorialhttp:_RareMETAL|//samtools.sourceforge.net/tabix.shtml '''TUTORIALtabix''']] with toy data are also available.indexed using the following commands:
bgzip study1.MetaScore.assoc tabix -s 1 -b 2 -e 2 -S 1 study1.MetaScore.assoc.gz tabix -s 1 -b 2 -e 2 -S 1 study1.MetaCov.assoc.gz ===== List of Studies =====* --studyName summaryFiles option is crucial for '''Rare MetalRAREMETAL''' to work. Ignoring this option will would lead to FATAL ERROR and '''Rare MetalRAREMETAL''' will would stop.
* The file should contain the path and prefix of the studies you want to include.
* If there is one or more studies that you want to excluded from your list, but want to save some effort of generating a new file, you can put a "#" in front of the line of record. '''Rare MetalRAREMETAL''' will would automatically exclude that study from meta analysis.* An example list of summary file is in the following:
/net/fantasia/home/sfengsph/prj/raremetal/raremetal/bin/META/TwinsUK/TwinsUK.TG.singlevar.score.txt.gz #/net/fantasia/home/sfengsph/prj/raremetal/raremetal/bin/META/HUNT/RareMetalWorker/HUNT_MI_case.TG.singlevar.score.txt.gz
* The above example study name file guides '''Rare Metal''' looking for the following files as input (note that the second study has been opt out from the meta When gene-level analysisis requested, because of --covFiles option should be used to specify the "#" in front of the line)covariance files. An example file is:
/net/fantasia/home/sfengsph/prj/raremetal/raremetal/bin/META/TwinsUK/TwinsUK.TG.singlevar.scorecov.txt.gz #/net/fantasia/home/sfengsph/prj/raremetal/raremetal/bin/META/TwinsUKHUNT/RareMetalWorker/TwinsUKHUNT_MI_case.TG.singlevar.cov.txt.gz
==== Grouping of Rare Variants====* The above example study name file guides '''RAREMETAL''' to look for summary statistics from TwinsUK study only, because "HUNT" study is commented out. The following two files are needed for '''RAREMETAL''' to perform further analysis together with their tabix index file are needed.
* Please sepcify --dosage option if input files were generated from dosage instead of genotype. =====Group Rare Variants===== ====== From a Group File ======
* Grouping methods are only necessary when doing gene-based or group-based burden tests in meta-analysis.
* If none of the grouping method is specified, then only single variant meta-analysis will be performed.
C1orf159 1:1021285:G:T 1:1021302:T:C 1:1021315:A:C 1:1021386:G:A 1:1022534:C:T 1:1025751:C:T 1:1026913:C:T
====== From an Annotated VCF File ======* If --groupFile option is '''NOT''' specified, '''RAREMETAL''' will look for an annotated vcf file as blue print for variants to group. Users are also allowed to generate a vcf file based on the superset of variants from pooled samples, and annotate outside RAREMETAL. Then, annotated vcf file can be used as input for RAREMETAL for gene-level meta-analysis, or group files can be generated based on the annotated vcf file. Detailed description of these options are [[Rare-Metal#Group_Rare_Variants_from_Annotated_VCF|'''available''']]. There are also [[Rare-Metal#Example_Command_lines|'''examples''']] of this usage at the bottom of this page. ==== QC Options ====* '''RAREMETAL''' allows filtering of variants from individual studies by their HWE pvalue and call rate, which are generated as part of the output from '''RAREMETALWORKER''' or [http://genome.sph.umich.edu/wiki/Rvtests '''rvtests'''].* To filter by HWE p-values, --hwe option should be used. The default is 0.0, which means not filtering any of the variants.* To filter by call rate, --callRate option can be specified. The default is 0.0, which allows no filtering utilized. ==== Association Options====* Currently, CMC type burden test, Madsen-Browning burden test, Variable Threshold burden test and SKAT are provided in '''RAREMETAL''', by specifying --burden, --MB, --VT and --SKAT.* --maf specifies the minor allele frequency cutoff when doing gene-based or group-based burden tests. Variants with maf '''above''' this threshold will be ignored. The default is maf<0.05.* In '''a single study''' of sample size N, if a site is monomorphic or not reported in vcf/ped, it is considered that the sample size of this study is not large enough to sample the rare allele. Thus, this study contributes 2*N reference alleles and 0 alternative allele towards meta-analysis. To let such studies contribute no alleles towards pooled allele frequency, specify --altMAF. ==== Conditional Analysis====* To decide whether a signal is caused by shadowing a significant common variant nearby, '''RAREMETAL''' also enables conditional analysis with a list of variants to be conditioned upon provided in a file as input for --condition option. An example input file should be space or tab delimited as in the following. When alleles do not match the ref and alt alleles from samples, the variant will be skipped from conditional analysis.  1:861349:C:T 1:905901:G:A 20:986998:G:C 22:3670691:A:G == Additional Analysis Options == === Generate a VCF File to Annotate Outside RAREMETAL ===* --writeVCF allows user to write a VCF file including pooled single variants from all studies. Then users can use their favorite annotation tool to annotate the VCF file. After annotating the VCF file, users can use that file as input for '''RAREMETAL''' for further gene-based or region-based meta analysis.* The output vcf file will be name as: yourPrefix.pooled.variants.vcf. An example output vcf file is in the following: #CHROM POS ID REF ALT QUAL FILTER INFO 1 115658497 115658497 G A . . ALT_AF=0.380906; 2 74688884 74688884 G A . . ALT_AF=8.33611e-05; 3 121414217 121414217 C A . . ALT_AF=0.0747833;===Annotation===* RAREMETAL automatically recognizes the annotation format generated by [[TabAnno | '''ANNO''']] or [[EPACTS#Annotating_VCF_file_using_EPACTS | '''EPACTS''']].* To annotate a the VCF generated in previous step, you can use the following command: ./anno --in your.in.vcf.gz --out your.out.vcf.gz === Group Rare MetalVariants from Annotated VCF ===* If --groupFile option is '''NOT''' specified, '''RAREMETAL''' will look for an annotated vcf file as blue print for variants to group.
* The annotated VCF file should be specified using --annotatedVcf option.
* --annotation should be used with --annotatedVcf together when specific category of functional variants are of interest to be grouped. For example, if grouping nonsynonymous and splicing variants are of interests, the following should be included in command line:
* (! only available after v4.13.8) when --annotation is not specified, raremetal groups all non-intergenic variants.
--annotatedVcf your.annotated.vcf --annotation nonsyn/splicing
* Notice that each variant is allowed to have more than one annotations; but each annotation should start with a new key "ANNO=" followed by annotation:genename:other transcript information.
* Generated group file will be named test.groupfile under your running directory.
===== Generate a VCF File to Annotate Outside of Rare Metal =====* --writeVCF allows user to write a VCF file including pooled single variants from all studies. Then users can use their favorite annotation tool to annotate the VCF file. After annotating the VCF file, users can use that file as input Options for '''Rare Metal''' for further gene-based or region-based meta analysis.* The output vcf file will be name as: yourPrefix.pooled.variants.vcf. An example output vcf file is in the following: #CHROM POS ID REF ALT QUAL FILTER INFO 1 115658497 115658497 G A . . ALT_AF=0.380906; 2 74688884 74688884 G A . . ALT_AF=8.33611e-05; 3 121414217 121414217 C A . . ALT_AF=0.0747833; ==== QC Options =Report Generation===* '''Rare Metal''' allows filtering of variants from individual studies by their HWE pvalue and call rate, which are generated as part of the output from '''Rare Metal Worker'''.* To filter by HWE p-values, --hwe option should be used. The default is 0.0, which means not filtering any of the variants.* To filter by call rate, --callRate option can be specified. The default is 0.0, which allows no filtering utilized. ==== Association Methods ====* Currently, four methods are provided in '''Rare Metal''', CMC type burden test, Madsen-Browning burden test, Variable Threshold burden test, correctGC generates QQ plots and SKATmanhattan plots with pvalues corrected using genomic control== Additional Analysis Options ==
* --prefix allows customized prefix for output files.
* --maf specifies the minor allele frequency cutoff when doing gene-based or group-based burden tests. The default is maf<0.05.
* --longOutput allows users to output not only burden test results but also the single variant results (allele frequencies, effect sizes, and p-values) for the variants being grouped together. Please refer to the output files section for detailed explanation and examples.
* --tabulateHits works with --hitsCutoff together to generate reports for genes that have p-value less than specified cutoff from burden tests or SKAT. The default cutoff of p-value for genes to be reported is 1.0e-06, which can be specified by --hitsCutoff option. For more explanations and examples, please go to [[http://genome.sph.umich.edu/wiki/Rare-Metal#Tabulated_Top_Hits TABULATED_HITS| Tabulated Top Hits]]. == Input Files ==Rare Metal needs the following as input:
==== List of Studies =Miscellaneous Options===* A file with the path and name --tabix allows rapid analysis when number of groups/genes of files containing summary statistics generated by raremetalworker should be specifiedinterests are small.* If no such file Currently, when number of groups is providedless than 100, '''Rare Metal''' will stop and report FATAL ERROR.* Please go to [[http://genome.sph.umich.edu/wiki/Rare-Metal#List_of_Studies_2 example input for study names]] for detailed explanation and examples-tabix option is automatically turned on.
==== Groups of Variants ====To perform gene-based or group-based burden test, groups of variants need to be provided. There are two options to provide such information: =====From Group File =====* A group file contains the list of groups or genes with the variants to be included in your burden tests. * Please refer to the instruction of --groupFile option for formats and examples. =====From Annotated VCF File =====* '''rareMETAL''' allows user to use annotated VCF file as input for grouping of variants, which is optional to input a group file as described above.* '''rareMETAL''' also has the option of generating a VCF file according to the pooled information from individuals studies. Then user can use their favorite annotation tools to annotate the VCF file into the INFO field. Currently, '''rareMETAL''' only support limited formats of annotated VCF file. * A more flexible way, which is also a recommended way, is to generate a group file from the customized annotated VCF file and use that as input to '''rareMETAL'''.* For formats of annotated VCF that '''rareMETAL''' currently support, please refer to the following [http://genome.sph.umich.edu/wiki/Rare-Metal#Grouping_from_an_Annotated_VCF_File annotated VCF]: '''NOTE:''' if no grouping method is provided, then only single variant meta analysis will be performed.     == Results Reported Reports Generated by rareMETAL RAREMETAL ==
=== Single Variant Meta Analysis Output ===
==== TABLES ====
* Single variant meta analysis output has the following components: header , results and resultsfootnote.
* Header lines start with "##" shows summary of the meta analysis including method used, number of studies, and total sample size.
* Header line starts with "#" are column headers for results table.
* Footnote also starts with "#", where genomic controls from each study and the overall sample are reported.
* An example single variant meta analysis output is shown below:
EFFECT_SIZE: Alternative Allele Effect Size
DIRECTION_BY_STUDY: Effect size direction of alternative allele from each study.
The order of study is consistent with the order of studies listed in the input file for option --studyNamesummaryFiles.
"?" means the variant is not observed or monomorphic from the study.
"!" means the variant observed from this study has different alleles from those in the first study.
==== PLOTS====
'''rareMETALRAREMETAL''' generates QQ plots and manhattan plots from single variant meta-analysis by default. Three QQ plots are generated, one with all variants included, one of variants with maf<0.05 and one of variants with maf<0.01. All plots are saved in a pdf file named yourPrefix.meta.plots.pdf. Genomic controls are also reported in the title of plots. When --correctGC option is specified, GC corrected plots are also generated.{| border="1" cellpadding="5" cellspacing="0" align="center"
|-
| align="center" width="100" | [[File:HDL.qq.plotQQ.png]]
|-
| align="center" width="200" | [[File:Singlevar.manhattanSingle_var_manhattan.png]]
|}
=== Burden Gene-level Tests Meta -Analysis Output ===When --longOutput is specified, output includes both burden test results of genes and single variant results of the variants included in burden tests. Otherwise, single variant results of variants included in burden tests will not be included in the output.
==== LONG TABLES ====
* When --longOutput is used, output includes both burden test results of genes and single variant results of the variants included in burden tests. Here is an example of output file from SKAT when --longOutput is specified.
##Method=Burden
##STUDY_NUM=2
==== SHORT TABLES ====
* Otherwise, single variant results of variants included in burden tests will not be included in the output. Here is an example of output file from SKAT when --longOutput is not specified.
##Method=Burden
* According to the example above, PCSK9 had a p-value of 7.54587e-11 from the gene-based burden test, where three variants from this gene were included. Another hit from this meta analysis is APOE, where only one variant was included in the burden test.
==== QQ PLOTS and MANHATTAN PLOTS ===='''rareMETALRAREMETAL''' generates QQ plots and manhattan plots from single variant and gene-level meta-analysis by default. Example QQ plots and manhattan plots are:{| border="1" cellpadding="5" cellspacing="0" align="center"
|-
| align="center" width="200" | [[File:burden.manhattan.png]]
|}
==== Log File LOG ====
* A log file is automatically generated by '''Rare MetalRAREMETAL''' to save the parameters in effect. An example is in the following:
The following parameters are in effect:
Grouping Methods:
============================
--groupFile [genes.file] --annotatedVcf [../../groupvcf/bin/debug/nonsynonymous.vcf]
--annotation []
--writeVcf [OFF]
--SKAT [false]
--VT [false]
--condition [condition.file]
Other Options:
============================
--tabix [OFF]
--correctGC [ON]
--prefix [test]
--maf [0.05]
--tabulateHits [false]
--hitsCutoff [1e-06]
--dosage [false]
--altMAF [false]
==Example Command lines==
* Here is an example command line to do single variant meta analysis only:
./raremetal --studyName summaryFiles your.studyNamelist.file of.summary.files --prefix yourPrefix
* When you want to do all burden tests using a group file to specify which variants to group:
./raremetal --studyName summaryFiles your.studyNamelist.file of.summary.files --covFiles your.list.of.cov.files --groupFile your.groupfile --burden --MB --SKAT --VT --maf 0.01 --prefix yourPrefix
(NOTE: this will generate single variant meta analysis result and the short format output for burden test results.)
* Here is how to do all SKAT meta analysis using a group file and request a long format output together with tabulated hits:
./raremetal --studyName summaryFiles your.studyNamelist.file of.summary.files --covFiles your.list.of.cov.files --groupFile your.groupfile --SKAT --longOutput --tabulateHits --hitsCutoff 1.0e-07 --prefix yourPrefix
* Here is an example of adding QC filters to variants when doing meta analysis.
./raremetal --studyName summaryFiles your.studyNamelist.file of.summary.files --covFiles your.list.of.cov.files --groupFile your.groupfile --SKAT --longOutput --tabulateHits --hitsCutoff 1.0e-07 --hwe 1e-06 --callRate 0.98 --prefix yourPrefix
* Here is how to do the same thing but reading grouping information from an annotated VCF file:
./raremetal --studyName summaryFiles your.studyNamelist.file of.summary.files --covFiles your.list.of.cov.files --annotatedVcf your.annotated.vcf --annotation nonsyn/stop/splicing --SKAT --longOutput --tabulateHits --hitsCutoff 1.0e-07 --hwe 1e-06 --callRate 0.98 --prefix yourPrefix
* If you want to write a VCF file of pooled variants from all studies, annotate them using your favorite annotation program, and then come back to '''Rare MetalRAREMETAL''' with the annotate VCF file to do burden tests:
First, use the following command to write the VCF file:
./raremetal --studyName summaryFiles your.studyNamelist.file of.summary.files --writeVcf --prefix yourPrefix Second, annotate the VCF file using your favorite annotation program. (Annotated VCF file has to follow the format described here: [[http://genome.sph.umich.edu/wiki/Rare-Metal#Grouping_from_an_Annotated_VCF_File Group_Rare_Variants_from_Annotated_VCF|annotated VCF format]])
Third, use the following command to do meta analysis:
./raremetal --studyName summaryFiles your.studyNamelist.file of.summary.files --covFiles your.list.of.cov.files --annotatedVcf your.annotated.vcf --annotation nonsyn/splicing/stop --burden --MB --SKAT --VT --maf 0.01 --prefix yourPrefix ==Other Useful Info== * Summary specs can be found [[Summary Files Specification for RAREMETAL]]
==TUTORIAL==
* For a comprehensive tutorial of RareMetalWorker RAREMETALWORKER and RareMETAL RAREMETAL using example data sets, please go to the following:  [http://genome.sph.umich.edu/wiki/Tutorial:_RareMETAL '''RAREMETAL Tutorial'''] * For a brief tutorial of rvtests, please go to:  [http://genome.sph.umich.edu/wiki/Rvtests '''rvtests'''] ==CONTACT==
[http://genome.sph.Please email Andy Boughton (abought at umichdot edu) for questions.edu/wiki/Tutorial:_RareMETAL '''RareMETAL Tutorial''']
== Change Log ==* Version 0.0.1 released to U of M CSG group. (2/13/2013)* Version 0.0.1 released to public. (2/24/2013)* Version 0.1.0 released to public after fixing a few bugs, adding conditional analysis Also check [[Raremetal Incoming updates | '''Known issues and automatic graphing incoming update in next version''']] to the tool. (8/5/2013)see if your problem has been reported before
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