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'''You may want to learn about new and improved [[Minimac4]].'''
'''minimac''' is a low memory, computationally efficient implementation of the MaCH algorithm for genotype imputation. It is designed to work on phased genotypes and can handle very large reference panels with hundreds or thousands of haplotypes. The name has two parts. The first, "mini", refers to the modest amount of computational resources it requires. The second, "mac", is short hand for [[MaCH]], our widely used algorithm for genotype imputation.
* [[Minimac Diagnostics]] - Summary of diagnostics for imputation performance generated by minimac
*[http Imputation server] - We are looking running imputation (and pre-phasing) for beta-testersyou!
= Download =
A binary Linux (64 bit) version of minimac is available [ from here] and source code [ from here]
The current version of minimac should be stamped 2013.7.17 - if your version shows a different version number or date stamp when it runs, it is not current.
-- all variants (SNPs, InDels, SVs) in the reference VCF will be imputed - independent from the FILTER column setting
- improved performance (Thanks to David Hinds - see also [[minimac2]] for the full set of performance improvements)
=== Preparing Your Data ===
To get started, you will need to store your data in [[Merlin]] format pedigree and data files, one per chromosome. For details, of the Merlin file format, see the [ Merlin Tutorial].
Within each file, markers should be stored by chromosome position. Alleles should be stored in the forward strand and can be encoded as 'A', 'C', 'G' or 'T' (there is no need to use numeric identifiers for each allele).
|style=white-space:nowrap|<code>-d sample.dat</code>
| Data file in [ Merlin format]. Markers should be listed according to their order along the chromosome.
| <code>-p sample.ped</code>
| Pedigree file in [ Merlin format]. Alleles should be labeled on the forward strand.
| <code>--states 200</code>
| <code>--refHaps ref.hap.gz </code>
| Reference haplotypes (e.g. from [ MaCH download page])
| <code>--vcfReference </code>
=== Reference Haplotypes ===
Reference haplotypes generated by the 1000 Genomes project and formatted so that they are ready for analysis are available from the [ MaCH download page]. As of this writing, the most recent set of haplotypes are based on genotype calls were generated in May 2011 and are an interim analysis of Project's Phase I data.
=== Imputation quality evaluation ===
=== Additional Sources of Information ===
If the combination of MaCH and Minimac still runs too slowly for you, and you have access to a multi-processor compute cluster, you can look at [[ChunkChromosome]] page to learn how to conveniently split each chromosome into multiple segments that can be analyzed in parallel. For information on how to put the resulting chunks back together, see [[Ligate Minimac|this page]].
If you are especially interested in 1000 Genomes Imputation, then you should look at the [[Minimac: 1000 Genomes Imputation Cookbook]].
= Post-imputation Association Analysis =
== Quantitative Traits ==
Please use [ mach2qtl].
== Binary Traits ==
Please use [ mach2dat]. Versions 1.0.18 and above accommodate to minimac output.
= Reference =
If you use [[minimac, ]] or [[minimac2]] please cite:  Fuchsberger C, Abecasis GR, Hinds DA. minimac2: faster genotype imputation. Bioinformatics 2014 []
Howie B, Fuchsberger C, Stephens M, Marchini J, and Abecasis GR.

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