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**Likely caused by overlap in dye emission frequencies
 
**Likely caused by overlap in dye emission frequencies
 
*T Accumulation
 
*T Accumulation
**The fluorophores used for thymine show lower removal rate after each iteration and accumulate over the sequencing run
+
**The fluorophores used for thymine are not always removed properly after each iteration
 +
**Intensity of T signal increases across sequencing run
    
==Alta-Cyclic==
 
==Alta-Cyclic==
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