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* help - print usage  
 
* help - print usage  
 
* test OUTPUT_DIR - run the test example placing the output in a user specified OUTPUT_DIR.  No other options are required.  
 
* test OUTPUT_DIR - run the test example placing the output in a user specified OUTPUT_DIR.  No other options are required.  
* out_dir OUTPUT_DIR - directory for the output  
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* outdir OUTPUT_DIR - directory for the output  
 
** May also be specified via OUT_DIR in the configuration file  
 
** May also be specified via OUT_DIR in the configuration file  
 
** Required to be set either via command-line or configuration  
 
** Required to be set either via command-line or configuration  
 
* conf CONFIG_FILE - configuration file  
 
* conf CONFIG_FILE - configuration file  
* index_file INDEX_FILE_NAME - name of the index file  
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* list FASTQ_LIST_FILE_NAME - name of the fastq list file  
** May also be specified via INDEX_FILE in the configuration file  
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** May also be specified via FASTQ_LIST in the configuration file  
 
** Required to be set either via command-line or configuration  
 
** Required to be set either via command-line or configuration  
 
* ref_dir REFERENCE_DIR - value to set config key REF_DIR to, overriding other values, REF_DIR can then be used inside config files.  
 
* ref_dir REFERENCE_DIR - value to set config key REF_DIR to, overriding other values, REF_DIR can then be used inside config files.  
 
** May also be specified via REF_DIR in the configuration file  
 
** May also be specified via REF_DIR in the configuration file  
* fastq FASTQ_PATH - prefix path to the fastq files specified in the INDEX_FILE
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* ref_prefix REFERENCE_DIR - path to prepend to non-absolute REF paths.
** May also be specified via FASTQ in the configuration file  
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** May also be specified via REF_PREFIX in the configuration file
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* fastq_prefix FASTQ_PATH - prefix path to the fastq files specified in the FASTQ_LIST
 +
** May also be specified via FASTQ_PREFIX in the configuration file
 +
* base_prefix BASE_PATH - prefix path to the prepend to fastq/ref files without absolute paths
 +
** May also be specified via BASE_PREFIX in the configuration file  
 
* keepTmp - Do not remove the temporary files (removed by default)  
 
* keepTmp - Do not remove the temporary files (removed by default)  
 
** May also be specified via KEEP_TMP in the configuration file  
 
** May also be specified via KEEP_TMP in the configuration file  
* numcs N - Replace N with the number of samples that should be processed in parallel
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* keepLog - Do not remove the intermediate log files (removed by default)
* numjobs N - Replace N with the number of targets in each makefile that should be run in parallel  
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** May also be specified via KEEP_LOG in the configuration file
 
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* numjobs N - Replace N with the number of samples that should be processed in parallel
 +
* threads N - Replace N with the number of targets in each makefile that should be run in parallel  
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* dryrun - Create the Makefile, but do not run it
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* maxlocaljobs N - Replace N with the maximum number of jobs that can be run locally (no batchtype specified).  Default is 10.
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* batchtype TYPE - Tells GotCloud the specified batch type to send jobs to the client nodes
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** May also be specified via BATCH_TYPE in the configuration file
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** Can be: mosix, slurm, slurmi, pbs, sge, sgei
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* batchopts OPTS - Tells GotCloud the options to pass onto the batch system
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** May also be specified via BATCH_OPTS in the configuration file
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* noPhoneHome - disable the phone home logic
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* gotcloudroot DIR - Specifies an alternate path to other gotcloud files rather than using the path to the gotcloud/align.pl.
 
Note: Command-line options take priority over configuration file settings
 
Note: Command-line options take priority over configuration file settings
  

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