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== How Do I? ==
== How Do I? ==
===== Q: Map between a query/read sequence and a reference sequence to do things like looking up a base/quality at a given reference position or looking up a reference base for a given read position?=====
===== Q: Map between a query/read sequence and a reference sequence to do things like looking up a base/quality at a given reference position or looking up a reference base for a given read position? =====
* A: Use [[C++ Class: CigarRoller#Mapping Between Reference and Read/Query|CigarRoller::getQueryIndex & CigarRoller::getRefOffset]]
* A: Use [[C++ Class: CigarRoller#Mapping Between Reference and Read/Query|CigarRoller::getQueryIndex & CigarRoller::getRefOffset]]
===== Q: Read only portions of a sorted & indexed BAM file that fall within a specified region? =====
* A:
*# open a sorted and indexed BAM file for reading (<code>SamFile::OpenForRead</code>)
*# read the BAM Index file (<code>SamFile::ReadBamIndex</code>)
*# read the header (<code>SamFile::ReadHeader</code>)
*# set the region to be read (<code>SamFile::SetReadSection</code>)
*# Loop calling <code>SamFile::ReadRecord</code> until it returns false - indicating there are no more reads within this region.
These methods are found in [[C++ Class: SamFile]].
NOTE: ReadRecord will also return false on an error - use <code>SamFile::GetStatus()</code> to determine if it was an error or the end of the region. <code>GetStatus</code> returns SamStatus::NO_MORE_RECS for the end of the region.
* Q: How do I read multiple regions?
** A: Use the above solution, but loop on the SetReadSection/ReadRecord steps until all sections have been processed.
===== Q: Get additional information on this library? =====
* A: Email me, Mary Kate, at mktrost@umich.edu.
