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, 01:10, 18 June 2014
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| You now have a tab delimited align.2x.index file (a little simpler than yesterday). | | You now have a tab delimited align.2x.index file (a little simpler than yesterday). |
| + | |
| + | == Create your GotCloud Configuration File == |
| + | Now that you have your FASTQ info file created, we need to setup the configuration. |
| + | |
| + | '''If you updated your gotcloud.2x.conf file yesterday, you still need to do this step''' |
| + | * We updated a few settings to match 1000g |
| + | *# Updated the version of the genome reference |
| + | *# Updated to use BWA instead of BWA_MEM |
| + | *# Updated the version of BWA |
| + | |
| + | You can copy from my directory to your personal directory: |
| + | cp /home/mktrost/seqshop/inputs/gotcloud.2x.conf ~/personal/. |
| + | |
| + | You need to update the conf file to find your align.2x.index and ensure your output directory is setup. |
| + | |
| + | nedit ~/personal/gotcloud.2x.conf & |
| + | (or use your favorite editor) |
| + | * Change the <code>IN_DIR</code> line, replacing <code>YOUR_USER_NAME</code> with your user name. |
| + | * Everything else is configured already. |
| + | [[File:Gc2xconf.png]] |
| + | |
| + | You'll notice that this file is very similar to the one we have been using. |
| + | * Just a few modifications to run a new test on the whole genome |