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==Running Gotcloud on Flux==

==How to run Gotcloud on Flux==

===First, Configure GotCloud like you would anywhere else===

===First, Configure GotCloud like you would anywhere else===

5. Run gotcloud with zero jobs to generate a Makefile.

5. Run gotcloud with zero jobs to generate a Makefile.

: <code>/path/to/gotcloud/gotcloud snpcall --conf /path/to/configuration.conf --numjobs 0</code>

: <code style="background:#f0f0f0">/path/to/gotcloud/gotcloud snpcall --conf /path/to/configuration.conf --numjobs 0</code>

:* The newly generated Makefile will be located in the directory <code>OUT_DIR</code> that is specified in your configuration file.  It will be named <code>umake.snpcall.Makefile</code>.

:* The newly generated Makefile will be located in the directory <code>OUT_DIR</code> that is specified in your configuration file.  It will be named <code>umake.snpcall.Makefile</code>.

6. Make a new folder, where you'll run your jobs from.

6. Make a new folder, where you'll run your jobs from.

7. Make an email address to send your jobs' status to.  I recommend that you don't use your primary one.

7. Make an email address to send your jobs' status to.  It'll be hit by hundreds or thousands of emails, so I recommend that you don't use your main email address here. 

:* If you're in a hurry to finish your pipeline, you can find an email address that will text the emails to your phone. Only use that in the second script, though, so that you don't receive thousands of text messages!

8. Figure out the name of the Flux account that you're going to use.  You can see which Flux accounts you have access to by running <code>mdiag -u $USER</code> and looking at the list after <code>ALIST</code>.

8. Figure out the name of the Flux account that you're going to use.  You can see which Flux accounts you have access to by running <code style="background:#eee;white-space:nowrap">mdiag -u $USER</code> and looking at the list after <code>ALIST</code>.

9. Figure out how many processors you're going to use at once.  Run <code>mdiag -a YOU_FLUX_ACCOUNT</code>.  I recommend running <code>MAXPROC</code> + <code>MAXIJOB[USER]</code> many jobs.  <code>MAXPROC</code> is the number of processors on your account, and <code>MAXIJOB[USER]</code> is the number of jobs that can sit idle in the queue waiting to be run (often 20).

9. Figure out how many processors you're going to use at once.  Run <code style="background:#eee;white-space:nowrap">mdiag -a YOU_FLUX_ACCOUNT</code>.  I recommend running <code>MAXPROC</code> + <code>MAXIJOB[USER]</code> many jobs.  <code>MAXPROC</code> is the number of processors on your account, and <code>MAXIJOB[USER]</code> is the number of jobs that can sit idle in the queue waiting to be run (often 20).

:* This number will usually be between 20 and 1000.

10. Figure out which pipelines to run first.  They will run in the order glfN, vcfN, pvcfN, filtN, svmN, splitN, allN where N is the name of a chromosome (ie, 1-22 and maybe X and Y).  If you skip a step, the <code>make</code> will run it for you.  If you're confident you can go straight to the step allN.  If you want to babysit the jobs, do them one-at-a-time. Your MAKEFILE_TARGETS will need one for each chromosome, like <code>glf1 glf2 glf3 glf4 glf5 glf6 glf7 glf8 glf9 glf10 glf11 glf12 glf13 glf14 glf15 glf16 glf17 glf18 glf19 glf20 glf21 glf22 </code>.  Feel free to use the script <code>perl -e 'print "glf$_ " for 1..22'</code> to mitigate repetitive strain injuries.

10. Figure out which steps to run first.  The steps go in the order glfN, vcfN, pvcfN, filtN, svmN, splitN, allN where N is the name of a chromosome (ie, 1-22 and maybe X and Y).  If you skip a step, it's not a problem, because <code>make</code> will run it for you.  If you're confident that everything will work beautifully, you can go straight to the step <code>allN</code> (or just <code>all</code> as a shortcut).

:* For example, I used <code style="background:#eee;white-space:nowrap">glf1 glf2 glf3 glf4 glf5 glf6 glf7 glf8 glf9 glf10 glf11 glf12 glf13 glf14 glf15 glf16 glf17 glf18 glf19 glf20 glf21 glf22 </code> the first time I ran on Flux.  Then I ran <code>vcf1 vcf2...</code>, and on down the list until finally <code>all</code>.

:* Feel free to use the script <code style="background:#eee;white-space:nowrap">perl -e 'print "glf$_ " for 1..22'</code> to mitigate repetitive strain injuries.

11. Inside that new folder, make a new file named <code>pbs.options</code> that contains the following:

11. Inside that new folder, make a new file named <code>pbs.options</code> that contains the following:

  #PBS -j oe

  #PBS -j oe

12. Still inside that folder, create a script that you will submit to flux.  It should look like this:

12. Still inside that folder, create a script that you will submit to flux.  Let's name it <code>script_thats_in_charge.sh</code>.  It should look like this:

  #!/bin/sh

  #!/bin/sh

   

   

   

   

  echo "job ended with status $? at $(date)"

  echo "job ended with status $? at $(date)"