SeqShop: Sequence Mapping and Assembly Practical, June 2014

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Note: the latest version of this practical is available at: SeqShop: Sequence Mapping and Assembly Practical

  • The ones here is the original one from the June workshop (updated to be run from elsewhere)

Introduction

See the introductory slides for an intro to this tutorial.

Goals of This Session

  • What we want to learn
    • Basic sequence data file formats (FASTQ, BAM)
    •  How to generate aligned sequences that are ready for variant calling from raw sequence reads
    •  How to evaluate the quality of sequence data
    •  How to visualize sequence data to examine the reads aligned to particular genomic positions

Setup in person at the SeqShop Workshop

This section is specifically for the SeqShop Workshop computers.

If you are not running during the SeqShop Workshop, please skip this section.


Login to the seqshop-server Linux Machine

This section will appear redundantly in each session. If you are already logged in or know how to log in to the server, please skip this section

  1. Login to the windows machine
    • The username/password for the Windows machine should be written on the right-hand monitor
  2. Start xming so you can open external windows on our Linux machine
    • Start->Enter "Xming" in the search and select "Xming" from the program list
    • Nothing will happen, but Xming was started.
    • View Screenshot
    • Xming.png

  3. Open putty
    • Start->Enter "putty" in the search and select "PuTTY" from the program list
    • View Screenshot
    • PuttyS.png

  4. Configure PuTTY in the PuTTY Configuration window
    • Host Name: seqshop-server.sph.umich.edu
    • View Screenshot
    • Seqshop.png

    • Setup to allow you to open external windows:
      • In the left pannel: Connection->SSH->X11
        • Add a check mark in the box next to Enable X11 forwarding
        • View Screenshot
        • SeqshopX11.png

    • Click Open
    • If it prompts about a key, click OK
  5. Enter your provided username & password as provided


You should now be logged into a terminal on the seqshop-server and be able to access the test files.

  • If you need another terminal, repeat from step 3.

Login to the seqshop Machine

So you can each run multiple jobs at once, we will have you run on 4 different machines within our seqshop setup.

  • You can only access these machines after logging onto seqshop-server

3 users logon to:

ssh -X seqshop1

3 users logon to:

ssh -X seqshop2

2 users logon to:

ssh -X seqshop3

2 users logon to:

ssh -X seqshop4

Setup your run environment

This will setup some environment variables to point you to

  • GotCloud program
  • Tutorial input files
  • Setup an output directory
source /home/mktrost/seqshop/setup.txt
  • You won't see any output after running source
    • It silently sets up your environment

View setup.txt

Setup.png

Setup when running on your own outside of the SeqShop Workshop

This section is specifically for running on your own outside of the SeqShop Workshop.

If you are running during the SeqShop Workshop, please skip this section.

Download & Build GotCloud

If you do not already have GotCloud:

  • cd to where you want GotCloud installed (you can change this to any directory you want)
mkdir -p ~/seqshop
cd ~/seqshop/
  • download, decompress, and build the version of gotcloud that was tested with this tutorial:
wget https://github.com/statgen/gotcloud/archive/gotcloud.workshop.tar.gz
tar xvf gotcloud.workshop.tar.gz
mv gotcloud-gotcloud.workshop gotcloud
cd gotcloud/src
make
cd ../..

Remember the path to gotcloud/ that is what you will need to set your GC variable to.

Download the example data

Download and untar file containing the example data used in the practicals:

wget http://csg.sph.umich.edu//mktrost/seqshopExample.tar.gz
tar xvf seqshopExample.tar.gz

You will see the names of all the files included in the example data scrolling on the screen as they are unpacked from the tar file.

Setup your run environment

Environment variables will be used throughout the tutorial.

We recommend that you setup these variables so you won't have to modify every command in the tutorial.

  1. Point to where you installed GotCloud
  2. Point to where you installed the seqshop files
  3. Point to where you want the output to go
Using bash (replace the paths below with the appropriate paths):
export GC=~/seqshop/gotcloud
export SS=~/seqshop/example
export OUT=~/seqshop/output
Using tcsh (replace the paths below with the appropriate paths):
setenv GC ~/seqshop/gotcloud
setenv SS ~/seqshop/example
setenv OUT ~/seqshop/output

Examining GotCloud Align Input Files

Examining Raw Sequence Reads : FASTQs

FASTQ : standard file format provided to you by those who did the sequencing.

For more information on the FASTQ format, see: http://en.wikipedia.org/wiki/FASTQ_format

For this tutorial, we will use FASTQs for 4 1000 Genome samples

  • Subset of FASTQs - should map to chromosome 22 36000000-37000000
ls ${SS}/fastq/

There are 24 fastq files: combination of single-end & paired-end.

Can you tell which files are single-end and which are paired-end?
  • View answer:
    • Paired-end files have a _1.fastq or _2.fastq extension
    • This convention isn't mandatory, but something similar is common
    • Fastqsm.png


Look at a couple of FASTQs:

less -S ${SS}/fastq/HG00551.SRR190851_1.fastq

less is a Linux command that allows you to look at a file.

  • -S option prevents line wrap
  • Use the arrow (up/down/left/right) keys to scroll through the file
  • Use the space bar to jump down a page

Use 'q' to exit out of less

q
Do you remember the parts of a FASTQ?
  • No, remind me:
  • Fastq.png


Look at the paired read:

less -S ${SS}/fastq/HG00551.SRR190851_2.fastq 

Remember, use 'q' to exit out of less

q
Do you notice something in common?
  • View answer:
    • Paired-end reads have matching read names with a different extensions
    • This convention isn't mandatory, but something similar is common
    • Fastq3.png

Reference Files

Reference files can be downloaded with GotCloud or from other sources

For alignment, you need:

  1. Reference genome FASTA file
  2. VCF (variant call format) files with chromosomes/positions
    • dbsnp - used to skip known variants when recalibrating
    • hapmap - used for sample contamination/sample swap validation

Take a look at the chromosome 22 reference files included for this tutorial:

ls ${SS}/ref22
  • View Screenshot
  • RefDir.png

Let's read the reference FASTA file (all reference bases for the chromosome):

less ${SS}/ref22/human.g1k.v37.chr22.fa

Remember, use 'q' to exit out of less

q
Where is the reference sequence?
  • Answer:
    • The ends of a chromosome are 'N' - unknown bases
    • Let's look at 5 lines of the file starting at line 300,000
    •  tail -n+300000 ${SS}/ref22/human.g1k.v37.chr22.fa |head -n 5
      

      Fasta.png

If you want to access the FASTA file by position, you can use samtools faidx command

${GC}/bin/samtools faidx ${SS}/ref22/human.g1k.v37.chr22.fa 22:36000000 | less

or

${GC}/bin/samtools faidx ${SS}/ref22/human.g1k.v37.chr22.fa 22:36000000-36000100

GotCloud FASTQ Index File

The FASTQ index file is created by you to tell GotCloud about each of your FASTQ files:

  • Where to find it
  • Sample name
    • Each sample can have multiple FASTQs
    • Each FASTQ is for a single sample
  • Run identifier
    • For recalibration we need to know which reads were in the same run.

FASTQ Index Format:

  • Tab delimited
  • Starts with a header line
  • One line per single-end read
  • One line per paired-end read (only 1 line per pair).

Let's look a look at the index file I prepared for this tutorial:

less -S ${SS}/align.index 

Remember, use 'q' to exit out of less

q
Which samples had multiple runs?
  • Need a reminder of the format?
  • Fqindex.png

    • Note: in the screenshots, the fields are shifted into clear columns to make it easier to read
      • When you view the file, the fields will not line up in neat columns and it can be hard to read
  • Hard to read the index? Need a hint?
    • Use cut to extract just the MERGE_NAME & RGID fields
    • cut -f 1,4 ${SS}/align.index
      
  • Answer:
    • HG00553 & HG00640
    • They have multiple unique values in the RGID field
    • FqindexRG.png


How do you point GotCloud to your index file?

  • Command-line --index_file option
or
  • Configuration file INDEX_FILE setting.

The command-line setting takes precedence over the configuration file setting.

GotCloud Configuration File

This file is created by you to configure GotCloud for your data.

  • Default values are provided in ${GC}/bin/gotcloudDefaults.conf
    • Most values should be left as the defaults
  • Specify values in your configuration file as:
    • KEY = value
  • Use $(KEY) to refer to another key's value
  • If a KEY is specified twice, the later value is used
  • Does not have access to environment variables
  • '#' indicates a comment

Let's look at the configuration file I created for this test:

more ${SS}/gotcloud.conf

Use the space bar to advance if the whole file isn't displayed.

If your references are in a different path than what is specified, what would you change?
  • Answer:
    • You would change REF_DIR to the new path
    • GcConf.png

Run GotCloud Align

AlignDiagram.png

Now that we have all of our input files, we need just a simple command to run them

${GC}/gotcloud align --conf ${SS}/gotcloud.conf --numcs 2 --base_prefix ${SS} --outdir ${OUT}
  • ${GC}/gotcloud runs GotCloud
  • align tells GotCloud you want to run the alignment pipeline.
  • --conf tells GotCloud the name of the configuration file to use.
    • The configuration for this test was downloaded with the seqshop input files.
  • --numcs means to run 2 samples at a time.
    • How many you can run concurrently depends on your system.
  • --base_prefix tells GotCloud the prefix to append to relative paths.
    • The Configuration file cannot read environment variables, so we need to tell GotCloud the path to the input files, ${SS}
    • Alternatively, gotcloud.conf could be updated to specify the full paths
  • --out_dir tells GotCloud where to write the output.
    • This could be specified in gotcloud.conf, but to allow you to use the ${OUT} to change the output location, it is specified on the command-line

GcalignStart.png

This should take 1-3 minutes to run.

It should end with a line like: Processing finished in 133 secs with no errors reported

If you cancelled GotCloud part way through, just rerun your GotCloud command and it will pick up where it left off.

Inside GotCloud align, not only sequence alignment but also pre-processing of sequence data, including deduplication and base quality recalibration are performed along with quality assessment, as illustrated below.

Gotcloud align detail.png

Examining GotCloud Align Output

Let's look at the output directory:

ls ${OUT}

GcalignOutM.png

Quality Control Files

Let's take a look at our quality control output directory:

ls ${OUT}/QCFiles 

GcalignOutQCm.png

Sample Contamination/Swap

Check for sample contamination:

  • *.selfSM : Main output file containing the contamination estimate.
    • Check the 'FREEMIX' column for genotype-free estimate of contamination
        • 0-1 scale, the lower, the better
        • If [FREEMIX] >= 0.03 and [FREELK1]-[FREELK0] is large, possible contamination
    • See VerifyBamID: A guideline to interpret output files for more information
less -S ${OUT}/QCFiles/HG00551.genoCheck.selfSM

Remember, use 'q' to exit out of less

q
Is there evidence of sample contamination?
  • Answer:
    • No, FREEMIX = 0.00000 (<0.03)

    Contam1.png

QC Metrics

See: QPLOT: Diagnose sequencing quality for more info on how to use QPLOT results.

Let's look at some quality control metrics:

cat ${OUT}/QCFiles/HG00551.qplot.stats
What is the mapping rate & average coverage for HG00551?
  • Answer
    • 98.93% Mapped
    • 7.43 MeanDepth

    Qplots.png

Generate a pdf of quality metrics:

Rscript ${OUT}/QCFiles/HG00551.qplot.R

Examine the PDF:

evince  ${OUT}/QCFiles/HG00551.qplot.pdf&

It is ok if you see a warning message when opening evince. It should still open. If not, let me know. To close evince, just close the pdf window.

Does the Empirical vs reported Phred score look as good as we would like?
  • Answer
    • No, it is well above the line
    • This is due to the small region used for recalibration
    • Qplotpdf.png

    • Look at the PDF I produced when I ran the whole genome:
    • evince ${SS}/ext/HG00551.wg.qplot.pdf&
      

    Qplotpdfwg.png

BAM Files

Binary Sequence Alignment/Map (SAM) Format

Let's look at the BAMs (aligned reads that are ready for variant calling):

ls ${OUT}/bams

GcalignOutBAMm.png

Let's examine at the first 5 lines of the BAM file using samtools view:

${GC}/bin/samtools view -h ${OUT}/bams/HG00551.recal.bam|head -n 5
What are the chromosome and position of the first record in the BAM file?
  • Need a reminder of the format?
  • Bam.png

  • Answer
    • Chr 22, Pos: 16114122

    BamRec.png

Accessing BAMs by Position

BAM's are so big, what if we want to see a position part way through the file?

Add a region to the view command we used above. Let's find all reads that overlap positions 36907000-36907005:

${GC}/bin/samtools view -h ${OUT}/bams/HG00551.recal.bam 22:36907000-36907005
  • Just a few reads.

Let's visualize what reads in that area look like using samtools tview:

${GC}/bin/samtools tview ${OUT}/bams/HG00551.recal.bam ${SS}/ref22/human.g1k.v37.chr22.fa
  • Type ‘g’
    • Type 22:36907000
  • Type ‘n’ to color by nucleotide
  • Use the arrow keys to move around and look at the area.

Understanding the syntax:

  • '.' : match to the reference on the forward strand
  • ',' : match to the reference on the reverse strand
  • ACGTN : mismatch to reference on the forward strand
  • acgtn : mismatch to reference on the reverse strand
Do you see anything interesting?
  • Screenshot
    • We will have to remember this region when we run snpcall to see what it says.

    Tview.png

Other tview commands:

  • Type '?' for a help screen
  • Type 'q' to quit tview

Feel free to play around more and browse the BAM files.

Other tools for BAMs

We have developed a lot of tools that operate on BAM files.

See Software: BamUtil Tools for a list

  • Many operations:
    • diff : diff 2 BAM files
    • stats: per positions statistics
    • bam2Fastq : convert a BAM back to a FASTQ (how I created the fastqs for this tutorial)
    • Lots of others
  • Feel free to try some out
  • If you have any questions, let me know, I wrote most of them and am happy to help.

Logging Off

This section is specifically for the SeqShop Workshop computers.

If you are not running during the SeqShop Workshop, please skip this section.

To logout of seqshop-server, type:

exit

And close the windows.

When done, log out of the Windows machine.