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, 22:37, 29 August 2012
LASER Manual
LASER is xxxx. To use LASER, please read part1 and part2. Also example will guide you through a complete procedure of how to use laser to infer ancestry. If you have feedbacks, please see contact.
= Part 1: Prepare data =
Use samtools pileup
Use pileup2laser.py script
NOTE: you genotype should be forward strand, mapping quality filter and base quality filter should be appropriately set up.
Tip: You can generate summary of coverage files using scripts.
= Part 2: Procrustes analysis =
You will need to download software from Chaonglong...
= How to interpret your sults =
You results will be output as xxx.
An example of outputs are like below
XXX
= Example =
We provide a toy example (downloadble from: xxx)
The workflow of LASER analysis are explained step by step.
1. generate pileup
2. prepare laser input
3. run laser
= Contact =
Xiaowei Zhan (for data preparation)
Chaolong Wang (for using Laser and interpreting results)